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human normal hepatic cells  (ATCC)


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    ATCC human normal hepatic cells
    Human Normal Hepatic Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2009 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human normal hepatic cells/product/ATCC
    Average 98 stars, based on 2009 article reviews
    human normal hepatic cells - by Bioz Stars, 2026-04
    98/100 stars

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    A The diagram illustrates our small RNA sequencing pipeline. B The heatmap displays the quality of reads based on their length. The read quality mainly concentrated at 38, ranging from 1 to 45 nt. C This histogram provides a visual representation of the distribution of sRNA tag length. Aside from the primary peak indicating miRNA (19–25), the secondary peak corresponds to piRNA (24–30). D The donut plot and list show sRNA tag mapping and annotation results. piRNAs represented only 1–3% of sequenced sRNAs. E Volcano plots show the distribution of differentially expressed genes in the three HCC cell lines compared to normal liver cells. F The Venn diagram shows the DEG counts in each HCC cell line. G The Venn diagram shows the DEG counts in each set. H Histogram display reads count distribution in all cell lines in sRNA sequencing result. I qPCR showed decreased pir-hsa-216911 expression in HCC cell lines compared to normal liver cells; ***p < 0.001, ** p < 0.01 by student-T tests against piRNA expression levels in <t>HL7702.</t>
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    Image Search Results


    A The diagram illustrates our small RNA sequencing pipeline. B The heatmap displays the quality of reads based on their length. The read quality mainly concentrated at 38, ranging from 1 to 45 nt. C This histogram provides a visual representation of the distribution of sRNA tag length. Aside from the primary peak indicating miRNA (19–25), the secondary peak corresponds to piRNA (24–30). D The donut plot and list show sRNA tag mapping and annotation results. piRNAs represented only 1–3% of sequenced sRNAs. E Volcano plots show the distribution of differentially expressed genes in the three HCC cell lines compared to normal liver cells. F The Venn diagram shows the DEG counts in each HCC cell line. G The Venn diagram shows the DEG counts in each set. H Histogram display reads count distribution in all cell lines in sRNA sequencing result. I qPCR showed decreased pir-hsa-216911 expression in HCC cell lines compared to normal liver cells; ***p < 0.001, ** p < 0.01 by student-T tests against piRNA expression levels in HL7702.

    Journal: Cell Death Discovery

    Article Title: pir-hsa-216911 inhibit pyroptosis in hepatocellular carcinoma by suppressing TLR4 initiated GSDMD activation

    doi: 10.1038/s41420-024-02285-9

    Figure Lengend Snippet: A The diagram illustrates our small RNA sequencing pipeline. B The heatmap displays the quality of reads based on their length. The read quality mainly concentrated at 38, ranging from 1 to 45 nt. C This histogram provides a visual representation of the distribution of sRNA tag length. Aside from the primary peak indicating miRNA (19–25), the secondary peak corresponds to piRNA (24–30). D The donut plot and list show sRNA tag mapping and annotation results. piRNAs represented only 1–3% of sequenced sRNAs. E Volcano plots show the distribution of differentially expressed genes in the three HCC cell lines compared to normal liver cells. F The Venn diagram shows the DEG counts in each HCC cell line. G The Venn diagram shows the DEG counts in each set. H Histogram display reads count distribution in all cell lines in sRNA sequencing result. I qPCR showed decreased pir-hsa-216911 expression in HCC cell lines compared to normal liver cells; ***p < 0.001, ** p < 0.01 by student-T tests against piRNA expression levels in HL7702.

    Article Snippet: Human normal hepatic cell line HL7702 and hepatocellular carcinoma cell lines Huh7, HepG2, and Hep3B were obtained from the China Center for Type Culture Collection.

    Techniques: RNA Sequencing, Sequencing, Expressing

    The relative expression profile of the selected lncRNAs in CHB patients, HBV-associated LC patients, and HBV-associated HCC patients. Relative lncRNA expression levels in PBMC samples were detected by qRT-PCR. ( A ) ADAMTSL4-AS1, ( B ) AC067931, ( C ) SOCS2-AS1, ( D ) AL731577, ( E ) TPM-AS, ( F ) AL356356, ( G ) AC037198, ( H ) AP002026. The expression levels of related lncRNAs in liver tissues were detected by qRT-PCR. ( I ) ADAMTSL4-AS1, ( J ) AC067931, ( K ) SOCS2-AS1, ( L ) AL731577. Relative expression levels of lncRNAs were upregulated in four human HCC cells(Huh7, HepG2, Hep3B, HepG2.215) comparison with Lx2 cells determining by qRT-PCR. ( M ) ADAMTSL4-AS1, ( N ) AC067931, ( O ) SOCS2-AS1. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: The Potential of the lncRNAs ADAMTSL4-AS1, AC067931 and SOCS2-AS1 in Peripheral Blood Mononuclear Cells as Novel Diagnostic Biomarkers for Hepatitis B Virus-Associated Hepatocellular Carcinoma

    doi: 10.2147/JHC.S463804

    Figure Lengend Snippet: The relative expression profile of the selected lncRNAs in CHB patients, HBV-associated LC patients, and HBV-associated HCC patients. Relative lncRNA expression levels in PBMC samples were detected by qRT-PCR. ( A ) ADAMTSL4-AS1, ( B ) AC067931, ( C ) SOCS2-AS1, ( D ) AL731577, ( E ) TPM-AS, ( F ) AL356356, ( G ) AC037198, ( H ) AP002026. The expression levels of related lncRNAs in liver tissues were detected by qRT-PCR. ( I ) ADAMTSL4-AS1, ( J ) AC067931, ( K ) SOCS2-AS1, ( L ) AL731577. Relative expression levels of lncRNAs were upregulated in four human HCC cells(Huh7, HepG2, Hep3B, HepG2.215) comparison with Lx2 cells determining by qRT-PCR. ( M ) ADAMTSL4-AS1, ( N ) AC067931, ( O ) SOCS2-AS1. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.

    Article Snippet: LX2 (human normal hepatic stellate cell line) and HCC (Huh7, HepG2, Hep3B, and HepG2.215) cell lines (Procell, Wuhan, China) were cultured under a 5% CO 2 atmosphere at 37°C, using Dulbecco’s Modified Eagle Medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Biological Industries, Israel), 1% streptomycin, and 1% penicillin solution (Gibco).

    Techniques: Expressing, Quantitative RT-PCR, Comparison